anti cd16 32 2 4 g2 bioxcell (Bio X Cell)

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Bio X Cell anti cd16 32 2 4 g2 bioxcell
Anti Cd16 32 2 4 G2 Bioxcell, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd16+32/InVivoMAb+anti-mouse+CD16+CD32/pm42815471-295-0-2
Average 96 stars, based on 370 article reviews

anti cd16 32 2 4 g2 bioxcell - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Specific deletion of interleukin-1 beta in microglia improves acute outcome and modulates neurogenesis after ischemic stroke
Article Snippet: Brain single-cell suspensions were stimulated with 50 ng/ml bacterial lipopolysaccharides (LPS) (InvivoGen; tlrl-3pelps) in the presence of Brefeldin A (Biolegend) at 37°C or kept in RPMI 10% FBS at 4°C. .. After 3 h, cells were washed in PBS and incubated with antibody cocktails plus anti-CD16/32 (2.4G2; BioXcell) for 15-20 min at 4°C. .. Cells were stained with combinations of the following antibodies, which were obtained from eBioscience and BioLegend: CD11b (M1/70), TCR-b (H57-597), MHC-II (M5/114.15.2), Ly6G (1A8), CD45 (30-F11), B220 (RA3-6B2), Ly6C (HK1.4), CD163 (S15049I), IL-1α (ALF-161) and IL-1β (NJTEN3).

Article Title: One-step generation of TCR knock-in mice targeted to the TCRβ locus results in functional mature T lymphocytes
Article Snippet: .. Isolated lymphocytes were resuspended in 1X PBE (PBS 1X supplemented with 0.5% BSA (Sigma-Aldrich cat. no. A9647-500G), 1mM EDTA) and first incubated in 1 μg/ml of anti-CD16/32 (BioXCell, cat. No. BE0307) for 5 min at room temperature. ..

Article Title: Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responses
Article Snippet: Upon sample processing, splenocytes from CD40lg SrtA/Y CD4- cre OT-II mice were incubated using a cocktail of biotinylated antibodies targeting Ter119, CD11c, CD25, B220, NK1.1, and CD8, followed by negative selection using anti-biotin beads (Miltenyi), as per the manufacturer’s instructions. .. Single-cell suspensions were incubated with 1 μg/ml anti-CD16/32 (2.4G2, BioXCell) in PBS supplemented with 0.5% BSA and 2 mM EDTA (PBE) for 5 minutes at RT and then stained for cell surface markers at 4 °C for 20 minutes in PBS. .. For single-cell transcriptomic analysis, stained cells were further incubated with DNA-barcoded anti-biotin and sample hashtag (anti-MHC-I) antibodies (BioLegend) for 20 minutes in PBE, washed three times with PBE, and bulk-sorted.

Article Title: Tumor Genotype Dictates Mitochondrial and Immune Vulnerabilities in Liver Cancer
Article Snippet: Red blood cell lysis was performed with ACK lysis buffer (GIBCO). .. Cells were incubated for 5 min with 1 μg ml −1 of anti-CD16/32 (2.4G2, BioXcell) on ice for 5 min. .. Cells were washed with PBS and stained with appropriate surface marker antibodies for CD45 (Biolegend, 103115), TCR-beta (Biolegend, 109228) and CD8a (Biolegend, 100707) for 20 min at 4 °C.

Isolation:

Article Title: CXCR5 Signals Fine-Tune Dendritic Cell Transcription and Regulate T H 2 Development.
Article Snippet: .. Briefly, msLN cells isolated from Hp-infected B6 and Cxcr5−/− msLNs at 8 days p.i. were positively enriched using anti-CD11c beads (Miltenyi) in the presence of anti-CD16/32 (2.4G2 BioXCell). .. Purified OT-II.4get transgenic CD4+ T cells (2 × 105) were incubated with the MACS-enriched DC populations (4 × 104) in complete media in the presence of increasing concentrations of OVA(323–339) peptide (New England Peptide) for 4 days with or without 100 ng recombinant murine CHI3L1 protein (R&D Systems).

Single Cell:

Staining:

Article Title: Tolerance to ferroptosis facilitates lipid metabolism and pathogenic type 2 immunity in allergic airway inflammation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Gpx4 IDT Mm.PT.58.12622924.g Hprt IDT Mm.PT.39a.22214828(1) Software and algorithms Cutadapt (version 1.16) 92 N/A DESeq2 93 N/A featureCounts (version 1.6.2) 94 N/A FlowJoTM Software V10.10.0 Becton, Dickinson and Company; 2023 https://www.flowjo.com/ LEGENDplexTM Analysis Software Biolegend https://legendplex.qognit.com LipidXplorer 95 N/A MSConvert 96 N/A Prism Graphpad 10 GraphPad Software https://www.graphpad.com/scientific- software/prism/ Qlucore software Qlucore https://qlucore.com QuPath 97 N/A R environment 98 N/A Sciex Software SCIEX https://sciex.com/ STAR (version 2.6.0) 99 N/A Other LS Columns Miltenyi Biotec Cat# 130-042-401 MojoSortTM Streptavidin Nanobeads Biolegend Cat# 480016 ll OPEN ACCESS Article e5 Immunity 59, 1–17.e1–e9, February 10, 2026 Cell isolation from tissues and flow cytometry Lung tissues were diced prior to digestion with 0.25 mg/ml Liberase TL (Sigma-Aldrich) and 1 mg/ml DNase I (Sigma-Aldrich) at 37 ◦ C for 1 h. Single cell suspensions were obtained by passing the digested lung tissue through a 70 μm cell strainer and further purified using a 37.5% Percoll gradient (Sigma-Aldrich), followed by red blood cell lysis with ammonium-chloride-potassium (ACK) lysis buffer (Elabscience). .. Anti-CD16/32 (BioXcell) was used to block Fc receptors and cells were stained with fluorochrome conjugated antibodies against the following surface antigens: CD3, NK1.1, Ter119, CD11c, CD19, CD11b, Gr-1 (Lineage), Siglec-F, CD45, Thy1.2, TCRb, CD4 and ST2. .. Dead cells were excluded by staining with DAPI (Carl Roth) or fixable Zombie UV (Biolegend).

Labeling:

Article Title: Complement-producing adventitial fibroblasts form an IL-33 alarmin hub that maintains ILC2s during airway allergy.
Article Snippet: .. Cells were washed with PBS, labeled with Zombie Aqua live/dead dye (BioLegend) for 10 min at room temperature (RT), and blocked with 20 μg/mL anti-CD16/32 (BioXCell) for an additional 20 min at RT. ..

Blocking Assay:

other:

Article Title: Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responses.
Article Snippet: In brief This study dissects the individual contributions of the different components of mRNA vaccines to immune responses.. Nucleoside-modified mRNA promotes the production of type I interferons that act on dendritic cells, while lipid nanoparticles instruct discrete Tfh-cellinducing properties in dendritic cells.. Both components are required for optimal germinal center responses.

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